CS202210140_4
Mono_3 (CS202210140_4)
Hierarchy: CS202210140_463 > CS202210140_4
PURL: https://purl.brain-bican.org/taxonomy/CS202210140/CS202210140_neurons/CS202210140_4
Labelset: Cluster (Rank: 1)
Parent Cell Set: Miscellaneous (CS202210140_463)
Cell Ontology Term: monocyte (CL:0000576)
Rationale: We then analyzed differentially expressed genes between clusters (Fig. 1c and Supplementary Fig. 1). Cluster 1 displayed high expression of monocyte genes such as CTSS, FCN1, S100A9, LYZ, VCAN. Clusters 2 and 3 shared high expression of macrophage genes such as LGMN, CTSB, CD14, APOE, C1QB, MARCO, CD163, FCGR3A. Cluster 4 expressed high levels of monocyte and macrophage-related genes such as FCN1, S100A9, VCAN, S100A8, MAFB. Clusters 5 and 6 expressed monocyte-related genes such as FCN1, S100A9, VCAN, FCGR1A, FCGR1B, as well as DC genes including CD1C, FCER1A, IFITM2, CLEC10A, FCGR2B. (Tang-Huau et al., 2018). From https://singlecell.broadinstitute.org/single_cell/study/SCP424/single-cell-comparison-pbmc-data#study-visualize : VCAN is not expressed much in CD16+ monocytes; TYROBP from the 10X single-cell PBMC data; FCN1 may also mark dendritic cells; CD14 expression seems too low to use as a marker.
| Rationale DOIs |
|---|
| https://doi.org/10.1038/s41467-018-04985-0 |
| Marker Genes |
|---|
| TYROBP |
| FCN1 |
| LYZ |
Author annotation fields:
| Author annotation | Value |
|---|---|
| Cluster ID | 3 |
| Class auto_annotation | MONO |
| Neurotransmitter auto_annotation | None |
| Neuropeptide auto_annotation | NAMPT |
| Subtype auto_annotation | None |
| Transferred MTG Label | None |
| Top three regions | Thalamus: 21.9%, Pons: 16.1%, Basal forebrain: 13.9% |
| Top three dissections | Human PnEN: 8.9%, Human MoAN: 6.6%, Human PnRF: 3.8% |
| Top Enriched Genes | FCN1, CLEC12A, S100A4, TNFRSF1B, IFI30, LYZ, CYTIP, FGR, LILRB2, KYNU |
| Number of cells | 503.0 |
| DoubletFinder score | 0.033227744 |
| Total UMI | 2407.775348 |
| Fraction unspliced | 0.569641907 |
| Fraction mitochondrial | 0.051116823 |
| H19.30.002 | 195.0 |
| H19.30.001 | 112.0 |
| H18.30.002 | 196.0 |
| H18.30.001 | 0.0 |
| Fraction cells from top donor | 0.389662028 |
| Number of donors | 3.0 |
| subcluster_id | None |